Anti-NF-kB p65 antibody [EP2161Y] (ab76311)
![Anti-NF-kB p65 antibody [EP2161Y] (ab76311)](https://www.abcam.com/ps/products/76/ab76311/Images/ab76311-260619-ab76311KOWB.jpg "Anti-NF-kB p65 antibody [EP2161Y] (ab76311)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2161Y] to NF-kB p65
- Suitable for: WB, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-NF-kB p65 antibody [EP2161Y]
See all NF-kB p65 primary antibodies -
Description
Rabbit monoclonal [EP2161Y] to NF-kB p65 -
Host species
Rabbit -
Specificity
ab76311 detects both phosphorylated and non-phosphorylated versions of human NF-kB p65. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human NF-kB p65. The exact sequence is proprietary.
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Positive control
- HeLa, Daudi and Jurkat cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2161Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: NF-κB p65 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76311 observed at 70 kDa. Red - ab8245 loading control, observed at 37 kDa.
ab76311 was shown to specifically react with NF-κB p65 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when NF-κB p65 knockout samples were used. Wild-type and NF-κB p65 knockout samples were subjected to SDS-PAGE. ab76311 (NF-κB p65) and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)](https://www.abcam.com/ps/products/76/ab76311/Images/ab76311-265972-ab76311-HT29500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)Immunocytochemistry/Immunofluorescence analysis HT-29 (human colorectal adenocarcinoma) labelling NF-kB p65 with purified ab76311 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Western blot - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)](https://www.abcam.com/ps/products/76/ab76311/Images/ab76311.jpg)
Western blot - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)All lanes : Anti-NF-kB p65 antibody [EP2161Y] (ab76311) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 65 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
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![Anti-NF-kB p65 antibody [EP2161Y] (ab76311)](https://www.abcam.com/ps/products/76/ab76311/Images/ab76311-4-benefits-of-recombinant-antibodies.png)
Anti-NF-kB p65 antibody [EP2161Y] (ab76311)
