Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NF-kB p65 antibody [EP2161Y] (ab76311)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2161Y] to NF-kB p65
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-NF-kB p65 antibody [EP2161Y]
    See all NF-kB p65 primary antibodies

  • Description

    Rabbit monoclonal [EP2161Y] to NF-kB p65

  • Host species

    Rabbit

  • Specificity

    ab76311 detects both phosphorylated and non-phosphorylated versions of human NF-kB p65.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NF-kB p65. The exact sequence is proprietary.

  • Positive control

    • HeLa, Daudi and Jurkat cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)
    Western blot - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: NF-κB p65 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab76311 observed at 70 kDa. Red - ab8245 loading control, observed at 37 kDa.


    ab76311 was shown to specifically react with NF-κB p65 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when NF-κB p65 knockout samples were used. Wild-type and NF-κB p65 knockout samples were subjected to SDS-PAGE. ab76311 (NF-κB p65) and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)
    Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)

    Immunocytochemistry/Immunofluorescence analysis HT-29 (human colorectal adenocarcinoma) labelling NF-kB p65 with purified ab76311 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Western blot - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)
    Western blot - Anti-NF-kB p65 antibody [EP2161Y] (ab76311)
    All lanes : Anti-NF-kB p65 antibody [EP2161Y] (ab76311) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 65 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Anti-NF-kB p65 antibody [EP2161Y] (ab76311)
    Anti-NF-kB p65 antibody [EP2161Y] (ab76311)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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