Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)

Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2294Y] to NF-kB p65 (phospho S536) - BSA and Azide free
  • Suitable for: IP, Dot blot, WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Product image
Anti-6X His tag® antibody (ab137839)
Product image
Anti-Smac/Diablo antibody [8H5AA3] (ab110288)
Product image
Anti-Aurora A antibody [EP1008Y] - BSA and Azide free (ab232013)
Product image
Recombinant Human RPS3 protein (ab113158)

Overview

  • Product name

    Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free
    See all NF-kB p65 primary antibodies
  • Description

    Rabbit monoclonal [EP2294Y] to NF-kB p65 (phospho S536) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Dot blot, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, C6 and NIH/3T3 whole cell lysate treated with Calyculin A + TNF-alpha. IP: Daudi cell lysate treated with Calyculin A + TNF-alpha.
  • General notes

    ab239882 is the carrier-free version of ab76302 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239882 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP2294Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • NFkB
    • RelA (p65)
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Microbiology
    • Interspecies Interaction
    • Host Immune Response
    • Metabolism
    • Types of disease
    • Obesity
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • NFkB
    • RelA (p65)

Images

  • Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
    Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)

    ab76302 at 1/30 immunoprecipitating NF-kB p65 (phospho S536) in Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.

    Lane 1 (input): Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha (10µg)

    Lane 2 (+): ab76302 + Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76302 in Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.

    For western blotting, ab76302 at 1/500 dilution (0.95 μg/ml) and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76302).

  • Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
    Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
    All lanes : Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 2 : C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 : C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

  • Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
    Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
    All lanes : Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) at 1/1000 dilution

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 30 minutes, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

  • Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
    Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
    All lanes : Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A and TNF-alpha whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

  • Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
    Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)

    Dot blot analysis of INF- kB p65 (phospho S536) phospho peptide (Lane 1) and NF- kB p65 non-phospho peptide (Lane 2) labeling NF-kB p65 (phospho S536) with ab76302 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76302).

  • Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
    Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)

  •  
  • Product image

    Anti-NF-kB p65 antibody [EP2161Y] (ab76311)

    Applications: Flow Cyt, ICC/IF, WB

  •  
  • Product image

    Anti-NF-kB p65 antibody [E379] (ab32536)

    Applications: ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Alexa Fluor® 647 Anti-NF-kB p65 antibody [E379] (ab190589)

    Applications: ICC/IF

  •  
  • Product image

    Anti-NF-kB p65 (phospho S529) antibody [EPR2379] (ab109458)

    Applications: Flow Cyt, IP, WB

  •  
  • Product image

    Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    Applications: ICC, IHC-P, IP, WB

  •  
  • Product image

    Anti-NF-kB p65 (phospho S276) antibody [EPR17622] (ab183559)

    Applications: ICC/IF, IP, WB

  •  
  • Product image

    Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] (ab190205)

    Applications: ICC/IF

Clear all

Recently viewed products

  •  
  • Product image

    Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)

  •  
  • Product image

    Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330)

  •  
  • Product image

    Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088)

  •  
  • Product image

    Anti-TIM 1 antibody [3A12E10] (ab233714)

  •  
  • Product image

    Anti-ATE1 antibody [EPR13667(2)] - BSA and Azide free (ab232619)

  •  
  • Product image

    Human TNF beta ELISA Kit (ab229202)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.