Rat IL-17F ELISA Kit (ab239423)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2 pg/ml
- Range: 9.4 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IL-17F ELISA Kit
See all IL-17F kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% splenocyte 8 2.3% Inter-assay Sample n Mean SD CV% splenocyte 3 2.1% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
9.4 pg/ml - 800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 91% - 115% Serum 89 87% - 92% Cell culture media 102 97% - 108% Hep Plasma 90 87% - 95% EDTA Plasma 122 112% - 130% Cit plasma 107 106% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat IL-17F ELISA Kit (ab239423) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17F protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-17F with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat IL-17F Capture Antibody 1 x 600µl 10X Rat IL-17F Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat IL-17F Lyophilized Recombinant Protein 2 vials Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation. -
Tissue specificity
Expressed in activated, but not resting, CD4+ T-cells and activated monocytes. -
Involvement in disease
Candidiasis, familial, 6 -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CANDF6
- Cytokine ML 1
- Cytokine ML-1
see all -
Database links
- Entrez Gene: 301291 Rat
- SwissProt: Q5BJ95 Rat
- Unigene: 34022 Rat
Images
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Other - Rat IL-17F ELISA Kit (ab239423)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Rat IL-17F Standard Curve ComparisonStandard Curve comparison between Rat IL-17F SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of rat IL-17F standard curve in Sample Diluent NS.The IL-17F standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of rat IL-17F standard curve in Sample Diluent 50BP.The IL-17F standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native IL-17F in rat treated and untreated rat splenocyte cell culture supernatant samples.Rat splenocyte cells were cultured in the presence (treated) or absence (untreated) of 10 ng/mL TPA and 10 ng/mL rat IL-1a for four days. The concentrations of IL-17F were measured in duplicates, interpolated from the IL-17F standard curves and corrected for sample dilution. Undiluted samples are as follows: treated splenocyte supernatant 5%, and untreated splenocyte supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17F concentration was determined to be 6,477 pg/mL in neat treated splenocyte supernatant, and 326.4 ng/mL in neat untreated splenocyte supernatant. -
Recombinant IL-17F homodimer, recombinant IL-17A homodimer, and recombinant IL-17A/F heterodimer proteins were measured with this kit.Background subtracted data values (mean +/- SD, n=2) are graphed. Note that the IL-17A protein is not detectable with this kit in the range assayed. -
Sandwich ELISA - Rat IL-17F ELISA Kit (ab239423)To learn more about the advantages of recombinant antibodies see here.
