Anti-Sec8 antibody - C-terminal (ab205945)
Key features and details
- Rabbit polyclonal to Sec8 - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Sec8 antibody - C-terminal
See all Sec8 primary antibodies -
Description
Rabbit polyclonal to Sec8 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human Sec8 aa 900 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q96A65 -
Positive control
- Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Sec8 antibody - C-terminal (ab205945)
Lane 1: Detection of Human Sec8 by Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab205945 at 6 µg for IP.
Lane 2: Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab205945 at 1 µg/ml. -
Western blot - Anti-Sec8 antibody - C-terminal (ab205945)All lanes : Anti-Sec8 antibody - C-terminal (ab205945) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells
Lane 2 : Whole cell lysate from 293T cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from mouse TCMK-1 cells
Lane 5 : Whole cell lysate from mouse NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 110 kDa
Exposure time: 30 seconds
