Anti-Sec8 antibody (ab224484)
Key features and details
- Rabbit polyclonal to Sec8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sec8 antibody
See all Sec8 primary antibodies -
Description
Rabbit polyclonal to Sec8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human Sec8 aa 800 to the C-terminus.
Database link: Q96A65 -
Positive control
- WB: RT4 and U-251 MG sp cell lysates. IHC-P: Human duodenum tissue. ICC/IF: U-251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Sec8 antibody (ab224484)All lanes : Anti-Sec8 antibody (ab224484) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 110 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sec8 antibody (ab224484)Paraffin-embedded human duodenum tissue stained for Sec8 using ab224484 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Sec8 antibody (ab224484)PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Sec8 (green) using ab224484 at 4 µg/ml in ICC/IF.
