Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21781] to NF-kB p65 (acetyl K310) - BSA and Azide free
  • Suitable for: Dot blot, ChIP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free
    See all NF-kB p65 primary antibodies

  • Description

    Rabbit monoclonal [EPR21781] to NF-kB p65 (acetyl K310) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 transfected with NF-kB p65 and p300 (aa1287-1663) expression vectors containing a myc-His-tag®, then treated with 20 ng/ml TNF-alpha for 60 minutes whole cell lysate.

  • General notes

    Ab237591 is the carrier-free version of ab218533. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab237591 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-NF-kB p65 antibody [EPR21781] - BSA and Azide free (ab237591)
    Western blot - Anti-NF-kB p65 antibody [EPR21781] - BSA and Azide free (ab237591)
    All lanes : Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533) at 1/2000 dilution

    Lane 1 : HEK-293 transfected with NF-kB p65 expression vector containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293 transfected with NF-kB p65 and p300 (aa1287-1663) expression vectors containing a myc-His-tag®, then treated with 20 ng/ml TNF-alpha for 60 minutes, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 60 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    NF-κB p65 (acetyl K310) expression is induced by TNF-α and p300 acetyltransferases (PMID: 20160011, PMID: 12456660, PMID: 16135789).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218533).

  • ChIP - Anti-NF-kB p65 antibody [EPR21781] - BSA and Azide free (ab237591)
    ChIP - Anti-NF-kB p65 antibody [EPR21781] - BSA and Azide free (ab237591)

    Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with and without 20 ng/ml TNF-α for 60 minutes according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 5 μg of ab218533 (red), and 20 µl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach).

    The ChIP data are consistent with the literature (PMID: 16135789).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218533).

  • Dot Blot - Anti-NF-kB p65 antibody [EPR21781] - BSA and Azide free (ab237591)
    Dot Blot - Anti-NF-kB p65 antibody [EPR21781] - BSA and Azide free (ab237591)

    Dot blot analysis of NF-kB p65 (acetyl K310) labeled with ab218533 at 1/1000 dilution.

    Lane 1: NF-κB p65 (acetyl K310) peptide.
    Lane 2: NF-κB p65 non-acetyl peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 2 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218533).

  • Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591)
    Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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