Anti-NF-kB p65 (phospho S536) antibody (ab28856)
Key features and details
- Rabbit polyclonal to NF-kB p65 (phospho S536)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NF-kB p65 (phospho S536) antibody
See all NF-kB p65 primary antibodies -
Description
Rabbit polyclonal to NF-kB p65 (phospho S536) -
Host species
Rabbit -
Specificity
NF-kappaB p65 (Phospho-Ser536) Antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at serine536. Suitable treatment will increase the expression - treatment with Calyculin A + TNF-alpha.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to NF-kB p65 (C terminal) (phospho S536).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NF-kB p65 (phospho S536) antibody (ab28856)All lanes : Anti-NF-kB p65 (phospho S536) antibody (ab28856)
Lane 1 : K562 cell lysate
Lane 2 : COLO cell lysate
Lane 3 : COLO cell lysate with Phospho Ser536 peptide
Predicted band size: 60 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S536) antibody (ab28856)Paraffin embedded human breast carcinoma stained with ab28856. Left: ab28856, Right: same antibody preincubated with the corresponding synthesized phosphopeptide.
