Anti-GYPB/GPB antibody [EPR15454(B)] (ab190351)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15454(B)] to GYPB/GPB
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-GYPB/GPB antibody [EPR15454(B)]
See all GYPB/GPB primary antibodies -
Description
Rabbit monoclonal [EPR15454(B)] to GYPB/GPB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human fetal liver, K562, mouse brain, kidney and spleen, rat brain, C6, Raw264.7, PC-12, and NIH/3T3 lysates.
-
General notes
This product was previously labelled as GYPB
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15454(B) -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-GYPB/GPB antibody [EPR15454(B)] (ab190351) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : C6 lysate
Lane 6 : Raw264.7 lysate
Lane 7 : PC12 lysate
Lane 8 : NIH/3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
-
Anti-GYPB/GPB antibody [EPR15454(B)] (ab190351) at 1/10000 dilution + K562 lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 10 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
-
Anti-GYPB/GPB antibody [EPR15454(B)] (ab190351) at 1/1000 dilution + Human fetal liver at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
-