Anti-Histone H2A (acetyl K74) antibody (ab241253)
Key features and details
- Rabbit polyclonal to Histone H2A (acetyl K74)
- Suitable for: ICC, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Histone H2A (acetyl K74) antibody
See all Histone H2A primary antibodies -
Description
Rabbit polyclonal to Histone H2A (acetyl K74) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H2A (acetyl K74).
Database link: P0C0S8 -
Positive control
- WB: HeLa and HepG2, treated with 30mM sodium butyrate for 4hr, whole cell lysates. ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4hr). ICC: HeLa cells (treated with 30mM sodium butyrate for 4hr).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
-
Western blot - Anti-Histone H2A (acetyl K74) antibody (ab241253)All lanes : Anti-Histone H2A (acetyl K74) antibody (ab241253) at 1/100 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 3 : HeLa whole cell lysate, untreated (-)
Lane 4 : HepG2 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K74) antibody (ab241253)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30mM sodium butyrate for 4hr) stained for Histone H2A (acetyl K74) (green) using ab241253 at 1/2.5 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor®488-conjugated Goat anti-Rabbit IgG(H+L).
-
Immunocytochemistry - Anti-Histone H2A (acetyl K74) antibody (ab241253)HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with 30mM sodium butyrate for 4hr) stained for Histone H2A (acetyl K74) using ab241253 at 1/5 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
