Anti-p21 antibody [HUGO291] (ab107099)
Key features and details
- Rat monoclonal [HUGO291] to p21
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-p21 antibody [HUGO291]
See all p21 primary antibodies -
Description
Rat monoclonal [HUGO291] to p21 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanWB Human -
Immunogen
Immunogen information is not available
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Positive control
- Mouse skin papilloma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
HUGO291 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse p21WT fibrosarcoma and mouse p21KO fibrosarcoma tissue sections labeling p21 with ab107099.
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All lanes : Anti-p21 antibody [HUGO291] (ab107099) at 1/50 dilution
Lane 1 : TT243 MEFs (non-irradiated) at 18.83 µg
Lane 2 : TT243 MEFs (irradiated with 10 Gy) at 20.8 µg
Lane 3 : p21-/- KO MEFs at 60 µg
Lane 4 : P21 WT MEFs at 75 µg
Lane 5 : HEK-V5-mp21 transfected cells at 20 µg
Lane 6 : HEK-V5-mp15 transfected cells at 20 µg
Predicted band size: 18 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hek-V5-mp21 transfected cells labelling p21 with ab107099. Nuclear staining was observed in Hek-V5-mp21 cytospins. Labeling with the anti-V5 mAb confirmed the efficiency of transfection. Cytospin preparation of human CD8 was used as a negative control.
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ab107099 at 1/100 staining p21 in conditional TRF1 knock out Mouse skin tissue by Immunohistochemistry.
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ab107099 at 1/100 staining p21 in Mouse skin papilloma tissue by Immunohistochemistry.