Anti-p21 antibody (ab227443)
Key features and details
- Rabbit polyclonal to p21
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p21 antibody
See all p21 primary antibodies -
Description
Rabbit polyclonal to p21 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human p21 (internal sequence). The exact sequence is proprietary.
Database link: P38936 -
Positive control
- WB: HCT 116 treated with cisplatin whole cell lysate; HeLa cell extract. IHC-P: SkHep1 xenograft tissue. ICC/IF: HeLa cells treated with cisplatin.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-p21 antibody (ab227443) at 1/1000 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) treated with 30 µM cisplatin for 0 hours, whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) treated with 30 µM cisplatin for 24 hours, whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 18 kDa12% SDS-PAGE gel.
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Paraffin-embedded SkHep1 xenograft tissue stained for p21 using ab227443 at 1/500 dilution in immunohistochemical analysis.
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4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated or treated with 30 µM cisplatin for 24 hours, stained for p21 (green) using ab227443 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).
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All lanes : Anti-p21 antibody (ab227443) at 1/500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : p21 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 18 kDa15% SDS-PAGE gel.