Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SDHA antibody [EPR9042(B)] (ab139181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9042(B)] to SDHA
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SDHA antibody [EPR9042(B)]
    See all SDHA primary antibodies

  • Description

    Rabbit monoclonal [EPR9042(B)] to SDHA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SDHA aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, fetal kidney, HepG2 and HeLa lysate; Human kidney and testis tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SDHA antibody [EPR9042(B)] (ab139181)
    Western blot - Anti-SDHA antibody [EPR9042(B)] (ab139181)
    All lanes : Anti-SDHA antibody [EPR9042(B)] (ab139181) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : SDHA knockout HEK-293 whole cell lysate
    Lane 3 : MCF7 whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab139181 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab139181 was shown to recognize SDHA in wild-type HEK-293 cells as signal was lost at the expected MW in SDHA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHA knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab139181 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SDHA antibody [EPR9042(B)] (ab139181)
    Western blot - Anti-SDHA antibody [EPR9042(B)] (ab139181)
    All lanes : Anti-SDHA antibody [EPR9042(B)] (ab139181) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 72 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] (ab139181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] (ab139181)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling SDHA with ab139181 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] (ab139181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] (ab139181)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling SDHA with ab139181 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] (ab139181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] (ab139181)

    Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab139181 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] (ab139181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9042(B)] (ab139181)

    Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab139181 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-SDHA antibody [EPR9042(B)] (ab139181)
    Anti-SDHA antibody [EPR9042(B)] (ab139181)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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