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Recombinant Human UBC3B protein (ab173060)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag N-Terminus
  • Suitable for: HPLC, SDS-PAGE

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Description

  • Product name

    Recombinant Human UBC3B protein
    See all UBC3B proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    ab173060 is greater than 95% pure, as determined by SEC-HPLC and reducing SDS-PAGE.
  • Endotoxin level

  • Expression system

    Escherichia coli
  • Accession

    Q712K3
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MAQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPN TLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPP VDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRK WRDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSND NSSDLLYDDLYDDDIDDEDEEEEDADCYDDDDSGNEES
    • Predicted molecular weight

      29 kDa including tags
    • Amino acids

      1 to 238
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Sequence for the tag: MGSSHHHHHHSSGLVPRGSH
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab173060 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      HPLC

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.50
      Constituents: 1.19% HEPES, 0.88% Sodium chloride

      ab173060 is supplied as an 0.2 µM filtered solution.

    General Info

    • Alternative names

      • CDC34B
      • E2 CDC34B
      • E2 ubiquitin conjugating enzyme R2
      • FLJ20419
      • MGC10481
      • UB2R2
      • UB2R2_HUMAN
      • UBC3B
      • UBE2R2
      • Ubiquitin carrier protein R2
      • Ubiquitin conjugating enzyme E2 CDC34B
      • Ubiquitin conjugating enzyme E2 R2
      • Ubiquitin conjugating enzyme E2R 2
      • Ubiquitin conjugating enzyme UBC3B
      • Ubiquitin protein ligase R2
      • Ubiquitin-conjugating enzyme E2 R2
      • Ubiquitin-conjugating enzyme E2-CDC34B
      • Ubiquitin-protein ligase R2
      see all
    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. May be involved in degradation of katenin.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Target information above from: UniProt accession Q712K3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab173060? Please let us know so that we can cite the reference in this datasheet.

    ab173060 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.50
      Constituents: 1.19% HEPES, 0.88% Sodium chloride

      ab173060 is supplied as an 0.2 µM filtered solution.

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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