Recombinant human IRE1b protein (ab190994)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant human IRE1b protein -
Biological activity
The specific activity of ab190994 ws determined to be 2.6 nmol/ min/ mg.
-
Purity
> 95 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
KQ AQPLDDPEAE QLTVVGKISF NPKDVLGRGA GGTFVFRGQF EGRAVAVKRL LRECFGLVRR EVQLLQESDR HPNVLRYFCT ERGPQFHYIA LELCRASLQE YVENPDLDRG GLEPEVVLQQ LMSGLAHLHS LHIVHRDLKP GNILITGPDS QGLGRVVLSD FGLCKKLPAG RCSFSLHSGI PGTEGWMAPE LLQLLPPDSP TSAVDIFSAG CVFYYVLSGG SHPFGDSLYR QANILTGAPC LAHLEEEVHD KVVARDLVGA MLSPLPQPRP SAPQVLAHPF FWSRAKQLQF FQDVSDWLEK ESEQEPLVRA LEAGGCAVVR DNWHEHISMP LQTDLRKFRS YKGTSVRDLL RAVRNKKHHY RELPVEVRQA LGQVPDGFVQ YFTNRFPRLL LHTHRAMRSC ASESLFLPYY PPDSEARRPC PGATGR -
Predicted molecular weight
80 kDa including tags -
Amino acids
499 to 926 -
Tags
proprietary tag N-Terminus -
Additional sequence information
NM_033266.
-
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSFThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
Functional Studies - Recombinant human IRE1b protein (ab190994)The specific activity of IRE1b (ab190994) was determined to be 2.4 nmol/min/mg as per activity assay protocol and was equivalent to 2.3 nmol/min/mg as per radiometric assay
-
SDS-PAGE - Recombinant human IRE1b protein (ab190994)SDS PAGE analysis of ab190994 -
SDS-PAGE - Recombinant human IRE1b protein (ab190994)SDS PAGE analysis of ab190994 -
Functional Studies - Recombinant human IRE1b protein (ab190994)The specific activity of ab190994 ws determined to be 2.6 nmol/ min/ mg.
-
SDS-PAGE - Recombinant human IRE1b protein (ab190994)The purity of ab190994 was determined to be >95% by densitometry, approx MW ~80 kDa.