Histone H3 Peptide Array Kit (96-well plate-based) (ab233494)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate
Overview
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Product name
Histone H3 Peptide Array Kit (96-well plate-based) -
Detection method
Colorimetric -
Assay duration
Multiple steps standard assay -
Product overview
The Histone H3 Peptide Array ELISA Kit (96-well plate-based) (ab233494) is designed to rapidly examine the selectivity and specificity of antibodies against 46 different histone modifications and is also suitable for identifying substrates of histone modifying enzymes and analyzing specificity of histone binding proteins.
In an assay with this kit, the Histone H3 proteins (modified at specific sites) that are tightly arrayed on the wells are incubated with input materials such as antibodies, proteins or enzymes. After incubation, the input materials are detected through an ELISA reaction system, the binding intensity is proportional to the intensity of the absorbance.
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Notes
Histone modifications have also been defined as epigenetic modifiers. Post-translational modifications of histones include the acetylation on specific lysine residues by histone acetyltransferases (HATs), the deacetylation by histone deacetylases (HDACs), the methylation of lysine and arginine residues by histone methytransferases (HMTs), the demethylation of lysine residues by histone demethylases (HDMTs), and the phosphorylation of specific serine groups by histone kinases (HKs). Next to DNA methylation, histone acetylation and histone methylation are the most well-characterized epigenetic marks. Generally, tri-methylation at H3-K4, H3-K36, or H3-K79 results in an open chromatin configuration and is, therefore, characteristic of euchromatin.
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Tested applications
Suitable for: PepArrmore details -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 28ml Adhesive Covering Film 1 unit Array Plate (96-well) 1 unit Developer Solution 1 x 12ml Stop Solution 1 x 10ml
Images
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Graphical analysis of specificity and binding intensity of H3K9me1 antibody.
The Histone H3 Array was probed with a H3K9me1 polyclonal antibody. Peptides and control were visualized using a goat anti-rabbit IgG-HRP secondary antibody.