Human DAPK3 (ZIP Kinase) knockout HEK-293T cell lysate (ab257407)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260223 - Human DAPK3 knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human DAPK3 (ZIP Kinase) knockout HEK293T cell lysate (ab257407)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: DAPK3 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab51602 Anti-ZIP Kinase antibody [EPR1636Y] was shown to specifically react with ZIP Kinase in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266755 (knockout cell lysate ab257407) lane below 53kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human DAPK3 (ZIP Kinase) knockout HEK293T cell lysate (ab257407)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: DAPK3 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab79422 observed at 53 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab79422 Anti-ZIP Kinase antibody [EPR1635] was shown to specifically react with ZIP Kinase in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266755 (knockout cell lysate ab257407) lane below 53kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab79422 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human DAPK3 knockout HEK293T cell lysate (ab257407)Homozygous: 1 bp insertion in exon 1