Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)
Key features and details
- Alexa Fluor® 594 Mouse monoclonal [2E3GC12FB2AE2] to SDHA
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2]
See all SDHA primary antibodies -
Description
Alexa Fluor® 594 Mouse monoclonal [2E3GC12FB2AE2] to SDHA -
Host species
Mouse -
Conjugation
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog -
Immunogen
Tissue, cells or virus corresponding to Cow SDHA. Purified mitochondrial complex II (Cow).
Database link: P31039 -
Positive control
- HeLa and HDFn cells.
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General notes
Product was previously marketed under the MitoSciences sub-brand.
ab170172 was previously used as a component in the MitoBiogenesis™ ICC Kit. The protocol for this kit is available here.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
2E3GC12FB2AE2 -
Isotype
IgG1 -
Research areas
Images
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ab170172 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab170172 at a 1/1000 dilution (shown in pseudo color red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
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Clone 2E3GC12FB2AE2 has been knock-out validated in its unconjugated form. Please refer to ab14715 datasheet for experimental details.
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Immunocytochemistry with HDFn (100x) cells.
A) HDFn stained with anti-SDHA Alexa-594 antibody (1.0 µg/mL). B) HDFn stained with Anti-mitochondrial chaperone HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria. -
Immunocytochemistry with HeLa cells (100x).
A) HeLa stained with anti-SDHA Alexa-594 antibody (1.0 µg/mL). B) HeLa stained with Anti-HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria.