Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)

Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [2E3GC12FB2AE2] to SDHA
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

You may also be interested in

Product image
Anti-XPB antibody [EPR14161] (ab190698)
Product image
Anti-C11orf79 antibody (ab243818)
Product image
Anti-Strumpellin antibody (ab126109)
Product image
PE Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab225508)

Overview

  • Product name

    Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2]
    See all SDHA primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [2E3GC12FB2AE2] to SDHA
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Full length native protein (purified) corresponding to Cow SDHA.

  • Positive control

    • HeLa and HDFn cells.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS, 30% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Purity is near homogeneity as judged by SDS-PAGE. ab154473 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality

    Monoclonal
  • Clone number

    2E3GC12FB2AE2
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex II

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)

    ab154473 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab154473 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)
    Western blot - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)

    Clone 2E3GC12FB2AE2 has been knock-out validated in its unconjugated form. Please refer to ab14715 datasheet for experimental details.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)
    Immunocytochemistry with HeLa cells (100x) were stained with anti-SDHA Alexa-488 antibody (1.0 µg/mL, ab154473) in green and DAPI in blue, as a nuclear stain.
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)
    Immunocytochemistry with HDFn (100x) cells were stained with Anti-SDHA Alexa-488 antibody (1.0 µg/mL, ab154473) in green, Anti-HSP60 (1/1000, ab46798) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-rabbit dyelight-594 (1/1000, ab96897).
  • Flow Cytometry - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)
    Flow Cytometry - Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)
    Flow Cytometry for Anti-SDHA Alexa-488 antibody (ab154473) stained HeLa cells. Flow cytometry results of unstained cells (black) and 0.2 µg/mL anti-SDHA Alexa-488 (red) stained cells.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)

  •  
  • Product image

    Anti-SDHA antibody (ab235594)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-SDHA antibody (ab137756)

    Applications: WB

  •  
  • Product image

    Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)

    Applications: Flow Cyt, ICC, IHC-Fr, IHC-P, WB

  •  
  • Product image

    Anti-SDHA antibody [EPR9042(B)] (ab139181)

    Applications: IHC-P, WB

  •  
  • Product image

    HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-SDHA antibody (ab209986)

    Applications: IHC-P, WB

  •  
  • Product image

    Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)

    Applications: ICC/IF, WB

  •  
  • Product image

    Anti-SDHA antibody [EPR9043(B)] - BSA and Azide free (ab240098)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-SDHA antibody [EPR9043(B)] (ab137040)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Natriuretic peptides A antibody [EPR20247] - BSA and Azide free (ab225873)

  •  
  • Product image

    Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449)

  •  
  • Product image

    Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free (ab249081)

  •  
  • Product image

    Anti-KDM5C / Jarid1C / SMCX antibody (ab264168)

  •  
  • Product image

    Anti-ERp57 antibody - C-terminal (ab192444)

  •  
  • Product image

    HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.