HRP Anti-CRISPR-Cas9 antibody [EPR18991] (ab215345)
![HRP Anti-CRISPR-Cas9 antibody [EPR18991] (ab215345)](https://www.abcam.com/ps/products/215/ab215345/Images/ab215345-282035-anti-crispr-cas9-antibody-epr18991-hrp-western-blot.jpg "HRP Anti-CRISPR-Cas9 antibody [EPR18991] (ab215345)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR18991] to CRISPR-Cas9
- Suitable for: WB
- Reacts with: Streptococcus pyogenes
- Conjugation: HRP
Overview
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Product name
HRP Anti-CRISPR-Cas9 antibody [EPR18991]
See all CRISPR-Cas9 primary antibodies -
Description
HRP Rabbit monoclonal [EPR18991] to CRISPR-Cas9 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Streptococcus pyogenes -
Immunogen
Recombinant fragment within Streptococcus pyogenes CRISPR-Cas9 aa 800-1000. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (Serotype M1).
Database link: Q99ZW2 -
Positive control
- WB: HEK-293T transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18991 -
Isotype
IgG
Images
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Western blot - HRP Anti-CRISPR-Cas9 antibody [EPR18991] (ab215345)All lanes : HRP Anti-CRISPR-Cas9 antibody [EPR18991] (ab215345) at 1/8000 dilution
Lane 1 : HEK-293T (Human embryonic kidney epithelial cell) transfected with vector control (pcDNA3.1(+)-EGFP-Myc), whole cell lysate
Lane 2 : HEK-293T transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate
Lane 3 : HEK-293T transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-Myc tag, whole cell lysate
Lane 4 : HEK-293T transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-Myc tag, whole cell lysate
Lane 5 : HEK-293T transfected vector control with GFP-Myc tag, whole cell lysate
Lane 6 : HEK-293T transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)) with Myc-His tag, whole cell lysate
Lane 7 : HEK-293T transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag, whole cell lysate
Lane 8 : HEK-293T transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)) with Myc-His tag, whole cell lysate
Lane 9 : HEK-293T transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag, whole cell lysate
Predicted band size: 158 kDa
Observed band size: 184 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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![HRP Anti-CRISPR-Cas9 antibody [EPR18991] (ab215345)](https://www.abcam.com/ps/products/215/ab215345/Images/ab215345-2-benefits-of-recombinant-antibodies.png)
HRP Anti-CRISPR-Cas9 antibody [EPR18991] (ab215345)
