HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493)
![HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493)](https://www.abcam.com/ps/products/198/ab198493/Images/ab198493-365980-ab198493-forpwsrectified.jpg "HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493)")
Key features and details
- HRP Mouse monoclonal [2E3GC12FB2AE2] to SDHA
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-SDHA antibody [2E3GC12FB2AE2]
See all SDHA primary antibodies -
Description
HRP Mouse monoclonal [2E3GC12FB2AE2] to SDHA -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Tissue, cells or virus corresponding to Cow SDHA. Purified mitochondrial complex II (Cow).
Database link: P31039 -
Positive control
- WB: Human heart mitochondrial tissue lysate. IHC-P: Normal human colon tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
2E3GC12FB2AE2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493)All lanes : HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493) at 1/5000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : SDHA knockout HEK-293 whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 70 kDaab198493 was shown to specifically react with SDHA in wild-type HEK-293 cells as signal was lost in SDHA knockout cells. Wild-type and SDHA knockout samples were subjected to SDS-PAGE. Ab198493 and ab181602 (Rabbit monoclonal to GAPDH - Loading Control loading) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493)](https://www.abcam.com/ps/products/198/ab198493/Images/ab198493-238825-IHCap22494761.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493)IHC image of SDHA staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198493, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Western blot - HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493)](https://www.abcam.com/ps/products/198/ab198493/Images/ab198493-244911-WBab1984931.jpg)
Western blot - HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493)All lanes : HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493) at 1/5000 dilution
Lane 1 : Human heart tissue lysate - mitochondrial extract (ab110337) at 10 µg
Lane 2 : Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198493 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
