Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23268-202] to Acetylcholinesterase
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Acetylcholinesterase antibody [EPR23268-202]
    See all Acetylcholinesterase primary antibodies

  • Description

    Rabbit monoclonal [EPR23268-202] to Acetylcholinesterase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain tissue lysate; Mouse and rat brain and striatum tissue lysates; HeLa, PC-12 and NIH/3T3 whole cell lysates. IP: Human and mouse brain tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23268-202

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)
    Western blot - Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)
    All lanes : Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human liver tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse striatum tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat striatum tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Human liver (PMID: 2400605).

    Exposure time: 3 minutes.

     

  • Immunoprecipitation - Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)
    Immunoprecipitation - Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)

    Acetylcholinesterase was immunoprecipitated from 0.35 mg human brain tissue lysate with ab253201 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253201 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human brain tissue lysate 10µg.

    Lane 2: ab253201 IP in human brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253201 in human brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

     

  • Western blot - Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)
    Western blot - Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)
    All lanes : Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane 1: 59 seconds; Lane 2: 26 seconds; Lane 3: 3 minutes.

     

  • Immunoprecipitation - Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)
    Immunoprecipitation - Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)

    Acetylcholinesterase was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab253201 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253201 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10µg.

    Lane 2: ab253201 IP in mouse brain tissue lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253201 in mouse brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6 seconds.

     

  • Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)
    Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201)

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