Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VASH1 antibody [EPR17420] (ab199732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17420] to VASH1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VASH1 antibody [EPR17420]
    See all VASH1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17420] to VASH1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HUVEC, A431, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, heart and kidney lysates; Rat brain and heart lysates; Human fetal kidney and fetal spleen lysates. ICC/IF: HUVEC cells. IP: A431 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17420

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-VASH1 antibody [EPR17420] (ab199732)
    Western blot - Anti-VASH1 antibody [EPR17420] (ab199732)
    All lanes : Anti-VASH1 antibody [EPR17420] (ab199732) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VASH1 antibody [EPR17420] (ab199732)
    Western blot - Anti-VASH1 antibody [EPR17420] (ab199732)
    All lanes : Anti-VASH1 antibody [EPR17420] (ab199732) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VASH1 antibody [EPR17420] (ab199732)
    Western blot - Anti-VASH1 antibody [EPR17420] (ab199732)
    All lanes : Anti-VASH1 antibody [EPR17420] (ab199732) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-VASH1 antibody [EPR17420] (ab199732)
    Immunoprecipitation - Anti-VASH1 antibody [EPR17420] (ab199732)

    VASH1 was immunoprecipitated from 1mg of A431 (Human epidermoid carcinoma) whole cell lysate with ab199732 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199732 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: A431 whole cell lysate 10 µg (Input). Lane 2: ab199732 IP in A431 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199732 in A431 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-VASH1 antibody [EPR17420] (ab199732)
    Anti-VASH1 antibody [EPR17420] (ab199732)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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