Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247)
![Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247)](https://www.abcam.com/ps/products/212/ab212247/Images/ab212247-455901-anti-p21-antibody-cip1-823-bsa-and-azide-free-western-blot-hela-human.jpg "Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247)")
Key features and details
- Mouse monoclonal [CIP1/823] to p21 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-p21 antibody [CIP1/823] - BSA and Azide free
See all p21 primary antibodies -
Description
Mouse monoclonal [CIP1/823] to p21 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human p21 aa 1 to the C-terminus. NCBI Accession No. 370771.
Database link: P38936 -
Positive control
- ICC: HeLa cells; WB: HeLa whole cell lysate; IHC-P: Skin, colon, breast carcinoma, lung squamous cell carcinoma, and bladder carcinoma tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
CIP1/823 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247)Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry - Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247)](https://www.abcam.com/ps/products/212/ab212247/Images/ab212247-3-anti-p21-antibody-cip1-823-bsa-and-azide-free-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247)Immunocytochemistry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling p21 with ab212247 followed by Goat anti-Mouse IgG-CF555 (Red). Membrane stained with Phalloidin 488 (Green). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247)](https://www.abcam.com/ps/products/212/ab212247/Images/ab212247-261671-ab212247IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247)Immunohistochemical analysis of formalix-fixed paraffin-embedded Human lung squamous cell carcinoma tissue, labeling p21 using ab212247 at 4 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247)](https://www.abcam.com/ps/products/212/ab212247/Images/ab212247-261670-ab212247IHC21.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247)Immunohistochemical analysis of formalix-fixed paraffin-embedded Human bladder carcinoma tissue, labeling p21 using ab212247 at 4 µg/mL.
