Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23630-49] to SCA2 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, ICC, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free
    See all SCA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23630-49] to SCA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293T, NIH/3T3 and PC-12 whole cell lysate. IHC-P: Human cerebrum tissue. Human, mouse and rat cerebellum tissue. IHC-Fr: Mouse cerebrum and cerebellum tissue. ICC: HeLa and NIH/3T3 cells. Flow Cyt: HeLa and NIH/3T3 cells.

  • General notes

    ab275752 is the carrier-free version of ab254362. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275752 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Immunocytochemistry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SCA2 with ab254362 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).

    The negative control is secondary antibody only.

  • Western blot - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Western blot - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    All lanes : Anti-SCA2 antibody [EPR23630-49] (ab254362) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 140 kDa
    Observed band size: 145,41 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 9989626).
    Lysates should be made freshly and used in WB immediately to minimize protein degradation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 10 seconds; Lane 4: 15 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SCA2 with ab254362 at 1/1000 dilution, followed by a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on neurons of human cerebrum (PMID: 9989626). The section was incubated with ab254362 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Flow Cytometry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SCA2 with ab254362 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Frozen sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Immunohistochemistry (Frozen sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling SCA2 with ab254362 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution. Positive staining on mouse cerebellum is observed. Nuclear counterstain is DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution. 

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling SCA2 with ab254362 at 1/1000 dilution, followed by a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat cerebellum (PMID: 26868665). The section was incubated with ab254362 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Flow Cytometry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling SCA2 with ab254362 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling SCA2 with ab254362 at 1/1000 dilution, followed by a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse cerebellum (PMID: 26868665). The section was incubated with ab254362 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Immunohistochemistry (Frozen sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling SCA2 with ab254362 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution. Positive staining on mouse cerebrum is observed. Nuclear counterstain is DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution. 

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling SCA2 with ab254362 at 1/1000 dilution, followed by a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on Purkinje cells in human cerebellum (PMID: 9989626). The section was incubated with ab254362 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Immunocytochemistry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

    This data was developed using ab254362, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling SCA2 with ab254362 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).

    The negative control is secondary antibody only.

  • Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)
    Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

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