Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free
See all SCA2 primary antibodies
Description

Rabbit monoclonal [EPR23630-49] to SCA2 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC	Mouse
IHC-Fr	Mouse
IHC-P	Mouse
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HEK-293T, NIH/3T3 and PC-12 whole cell lysate. IHC-P: Human cerebrum tissue. Human, mouse and rat cerebellum tissue. IHC-Fr: Mouse cerebrum and cerebellum tissue. ICC: HeLa and NIH/3T3 cells. Flow Cyt: HeLa and NIH/3T3 cells.
General notes
ab275752 is the carrier-free version of ab254362. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275752 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23630-49
Isotype

IgG
Research areas

Immunocytochemistry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SCA2 with ab254362 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).

The negative control is secondary antibody only.
Western blot - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

All lanes : Anti-SCA2 antibody [EPR23630-49] (ab254362) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 140 kDa
Observed band size: 145,41 kDa
why is the actual band size different from the predicted?

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

The molecular weight observed is consistent with what has been described in the literature (PMID: 9989626).
Lysates should be made freshly and used in WB immediately to minimize protein degradation.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-3: 10 seconds; Lane 4: 15 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SCA2 with ab254362 at 1/1000 dilution, followed by a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on neurons of human cerebrum (PMID: 9989626). The section was incubated with ab254362 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SCA2 with ab254362 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling SCA2 with ab254362 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at a 1/1000 dilution. Positive staining on mouse cerebellum is observed. Nuclear counterstain is DAPI.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at a 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling SCA2 with ab254362 at 1/1000 dilution, followed by a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat cerebellum (PMID: 26868665). The section was incubated with ab254362 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling SCA2 with ab254362 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling SCA2 with ab254362 at 1/1000 dilution, followed by a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse cerebellum (PMID: 26868665). The section was incubated with ab254362 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling SCA2 with ab254362 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at a 1/1000 dilution. Positive staining on mouse cerebrum is observed. Nuclear counterstain is DAPI.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at a 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling SCA2 with ab254362 at 1/1000 dilution, followed by a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on Purkinje cells in human cerebellum (PMID: 9989626). The section was incubated with ab254362 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary from Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunocytochemistry - Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)

This data was developed using ab254362, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling SCA2 with ab254362 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).

The negative control is secondary antibody only.
Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752)