Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12021-114] to IMP3 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free
    See all IMP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR12021-114] to IMP3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within IMP3 aa 1-200. The exact sequence is proprietary.
    Database link: O00425

  • Positive control

    • IHC-P: Human lung cancer and fetal kidney tissues. WB: HeLa, 293T and BxPC-3 cell lysates. HeLa, HEK-293 and BxPC-3 whole cell lysates.

  • General notes

    ab250113 is the carrier-free version of ab179807 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250113 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    Western blot - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    All lanes : Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 63 kDa
    Observed band size: 69 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab179807, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    This data was developed using ab179807, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling IMP3 with purified ab179807 at 1/1000 dilution (0.10 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    Western blot - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)

    This data was developed using ab179807, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - Unpurified ab179807 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

    Unpurified ab179807 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. ab179807 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)

    This data was developed using ab179807, the same antibody clone in a different buffer formulation.

    Unpurified ab179807 at 1:100 staining IMP3 antibody in human lung cancer tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling IMP3 with ab179807 at 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    Western blot - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    All lanes : Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 63 kDa
    Observed band size: 69 kDa why is the actual band size different from the predicted?



    This data was developed using ab179807, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)

    This data was developed using ab179807, the same antibody clone in a different buffer formulation.

    Unpurified ab179807 at 1:100 staining IMP3 antibody in human fetal kidney tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue labeling IMP3 with ab179807 at 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)

    This data was developed using ab179807, the same antibody clone in a different buffer formulation.

    Negative control: Immunohistochemical analysis of paraffin-embedded human adult kidney tissue labeling IMP3 with Unpurified ab179807 at 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)
    Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free (ab250113)

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