Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)
![Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)](https://www.abcam.com/ps/products/250/ab250277/Images/ab250277-403483-anti-nuf2-antibody-epr13019-bsa-and-azide-free-western-blot.jpg "Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13019] to Nuf2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-Nuf2 antibody [EPR13019] - BSA and Azide free
See all Nuf2 primary antibodies -
Description
Rabbit monoclonal [EPR13019] to Nuf2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250277 is the carrier-free version of ab180945. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250277 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13019 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)All lanes : Anti-Nuf2 antibody [EPR13019] (ab180945) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDaThis data was developed using ab180945, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)](https://www.abcam.com/ps/products/250/ab250277/Images/ab250277-1-anti-nuf2-antibody-epr13019-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)This data was developed using ab180945, the same antibody clone in a different buffer formulation.Lysate from 293 cells was immunoprecipitated with ab180945 at a 1/50 dilution. For the subsequent blot, ab180945 used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Second lane is the negative control. -
![Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)](https://www.abcam.com/ps/products/250/ab250277/Images/ab250277-3-benefits-of-recombinant-antibodies.png)
Anti-Nuf2 antibody [EPR13019] - BSA and Azide free (ab250277)