Anti-Ras (mutated G12D) antibody (ab221163)
Key features and details
- Rabbit polyclonal to Ras (mutated G12D)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ras (mutated G12D) antibody
See all Ras primary antibodies -
Description
Rabbit polyclonal to Ras (mutated G12D) -
Host species
Rabbit -
Specificity
G12D mutant specific. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human Ras (N terminal) (mutated G12D). (Carrier-protein conjugated).
Database link: P01111 -
Positive control
- Human colon cancer tissue; AsPC-1 whole cell extract; Ras (mutant G12D) transfected 293T whole cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ras (mutated G12D) antibody (ab221163)All lanes : Anti-Ras (mutated G12D) antibody (ab221163) at 1/5000 dilution
Lane 1 : Non-transfected 293T whole cell extract
Lane 2 : Ras wild type transfected 293T whole cell extract
Lane 3 : Ras mutated G12D transfected 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 21 kDa12% SDS-PAGE.
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Western blot - Anti-Ras (mutated G12D) antibody (ab221163)Anti-Ras (mutated G12D) antibody (ab221163) at 1/500 dilution + AsPC-1 whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 21 kDa12% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated G12D) antibody (ab221163)Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Ras (mutated G12D) with ab221163 at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated G12D) antibody (ab221163)Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Ras (mutated G12D) with ab221163 at 1/1000 dilution.
