Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸ 10 µl 134 016 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1125Y] to Ras
Suitable for: ICC/IF, WB, IP, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Ras antibody [EP1125Y]
See all Ras primary antibodies
Description

Rabbit monoclonal [EP1125Y] to Ras
Host species

Rabbit
Specificity

ab52939 is predicted to react with H-Ras, N-Ras and K-Ras.

Tested Applications & Species

Application	Species
Flow Cyt	Rat
ICC/IF	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Ras aa 1-100 (N terminal).
Positive control
- Flow Cyt: PC-12 cells. WB: Jurkat, 293T, RAW 246.7, Neuro-2a, PC-12 and C6 lysates; Human Ras full length protein. ICC/IF: MCF7 cells. IP: Jurkat whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1125Y
Isotype

IgG
Research areas

Western blot - Anti-Ras antibody [EP1125Y] (ab52939)

All lanes : Anti-Ras antibody [EP1125Y] (ab52939) at 1/5000 dilution (purified)

Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysates
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysates
Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysates
Lane 4 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] (ab52939)

Immunocytochemistry analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling Ras with Purified ab52939 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunoprecipitation - Anti-Ras antibody [EP1125Y] (ab52939)

ab52939 (purified) at 1:20 dilution (2μg) immunoprecipitating Ras in Jurkat whole cell lysate.

Lane 1 (input): Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate 10μg
Lane 2 (+): ab52939 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52939 in Jurkat whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-Ras antibody [EP1125Y] (ab52939)

Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma cell line) cells labeling Ras with purified ab52939 at 1:30 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-Ras antibody [EP1125Y] (ab52939)

Anti-Ras antibody [EP1125Y] (ab52939) at 1/5000 dilution (purified) + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 21 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-Ras antibody [EP1125Y] (ab52939)

Anti-Ras antibody [EP1125Y] (ab52939) at 1/500000 dilution (unpurified) + C6 (rat glial tumor cell line) cell lysate at 10 µg/ml

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 21 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-Ras antibody [EP1125Y] (ab52939)

Unpurified ab52939 at 1/100 dilution staining Ras in permeabilized PC-12 (rat adrenal gland pheochromocytoma cell line) cells by flow cytometry (red). Rabbit IgG negative control (green).
Western blot - Anti-Ras antibody [EP1125Y] (ab52939)

Anti-Ras antibody [EP1125Y] (ab52939) at 1/500 dilution (unpurified) + Human Ras full length protein (ab56526) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 21 kDa

Exposure time: 1 minute
Anti-Ras antibody [EP1125Y] (ab52939)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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