Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1125Y] to Ras - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ras antibody [EP1125Y] - BSA and Azide free
    See all Ras primary antibodies

  • Description

    Rabbit monoclonal [EP1125Y] to Ras - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: PC-12 cells. ICC/IF: MCF7 cells. IP: Jurkat whole cell lysate.

  • General notes

    ab271848 is the carrier-free version of ab52939. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)
    Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)

    Immunocytochemistry analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling Ras with Purified ab52939 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52939).
  • Immunoprecipitation - Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)
    Immunoprecipitation - Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)

    ab52939 (purified) at 1:20 dilution (2μg) immunoprecipitating Ras in Jurkat whole cell lysate.

    Lane 1 (input): Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate 10μg
    Lane 2 (+): ab52939 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52939 in Jurkat whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52939).
  • Flow Cytometry - Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)
    Flow Cytometry - Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)

    Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma cell line) cells labeling Ras with purified ab52939 at 1:30 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52939).
  • Flow Cytometry - Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)
    Flow Cytometry - Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)

    Unpurified ab52939 at 1/100 dilution staining Ras in permeabilized PC-12 (rat adrenal gland pheochromocytoma cell line) cells by flow cytometry (red). Rabbit IgG negative control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52939).
  • Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)
    Anti-Ras antibody [EP1125Y] - BSA and Azide free (ab271848)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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