Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3255] to Ras - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ras antibody [EPR3255] - BSA and Azide free
    See all Ras primary antibodies

  • Description

    Rabbit monoclonal [EPR3255] to Ras - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab108602 also detects H-Ras and N-Ras.

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T and SH-SY5Y cell lysates; Rat brain lysates; Mouse heart lysates IP: SH-SY5Y cell lysates Flow Cyt: HEK-293 cells ICC/IF: HEK-293T cells

  • General notes

    Ab209974 is the carrier-free version of ab108602. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab209974 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Ras with purified ab108602 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti ras antibody epr3255 immunocytochemistry 293t human)
  • Flow Cytometry - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Flow Cytometry - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Ras with purified ab108602 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108602)
  • Immunoprecipitation - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Immunoprecipitation - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    ab108602 (purified) at 1/20 dilution (2 µg) immunoprecipitating Ras in SH-SY5Y whole cell lysate.
    Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab108602 & SH-SY5Y whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108602 in SH-SY5Y whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108602)
  • Western blot - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Western blot - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    All lanes : Anti-Ras antibody [EPR3255] (ab108602) at 1/1000 dilution (unpurified)

    Lane 1 : HEK-293 whole cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 21 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108602).

  • Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)

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