Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1125Y] to Ras - Low endotoxin, Azide free
  • Suitable for: WB, Flow Cyt, IP, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free
    See all Ras primary antibodies

  • Description

    Rabbit monoclonal [EP1125Y] to Ras - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • C6 cell lysate and permeabilized PC-12 cells.

  • General notes

    ab140261 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)
    Immunoprecipitation - Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)

    ab52939 (purified) at 1:20 dilution (2μg) immunoprecipitating Ras in Jurkat whole cell lysate.

    Lane 1 (input): Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate 10μg
    Lane 2 (+): ab52939 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52939 in Jurkat whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52939).

  • Flow Cytometry - Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)
    Flow Cytometry - Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)

    Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma cell line) cells labeling Ras with purified ab52939 at 1:30 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52939).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)
    Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)

    Immunocytochemistry analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling Ras with Purified ab52939 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52939).

  • Flow Cytometry - Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)
    Flow Cytometry - Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)

    Unpurified ab52939 at 1/100 dilution staining Ras in permeabilized PC-12 (rat adrenal gland pheochromocytoma cell line) cells by flow cytometry (red). Rabbit IgG negative control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52939).

  • Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)
    Anti-Ras antibody [EP1125Y] - Low endotoxin, Azide free (ab140261)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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