Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARL2BP antibody [EPR15265] - BSA and Azide free (ab250866)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15265] to ARL2BP - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ARL2BP antibody [EPR15265] - BSA and Azide free
    See all ARL2BP primary antibodies

  • Description

    Rabbit monoclonal [EPR15265] to ARL2BP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human ARL2BP aa 1-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9Y2Y0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: A549, HepG2, HeLa, HUVEC and MCF7 cell lysate; Human fetal brain tissue lysate.

  • General notes

    Ab250866 is the carrier-free version of ab185964. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250866 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ARL2BP antibody [EPR15265] - BSA and Azide free (ab250866)
    Western blot - Anti-ARL2BP antibody [EPR15265] - BSA and Azide free (ab250866)
    All lanes : Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ARL2BP knockout HeLa cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 19 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab185964, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab185964 observed at 20 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab185964 Anti-ARL2BP antibody [EPR15265] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265269 (knockout cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab185964 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ARL2BP antibody [EPR15265] - BSA and Azide free (ab250866)
    Western blot - Anti-ARL2BP antibody [EPR15265] - BSA and Azide free (ab250866)
    All lanes : Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HUVEC lysate
    Lane 3 : A549 lysate
    Lane 4 : HepG2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa



    This data was developed using ab185964, the same antibody clone in a different buffer formulation.

     

  • Anti-ARL2BP antibody [EPR15265] - BSA and Azide free (ab250866)
    Anti-ARL2BP antibody [EPR15265] - BSA and Azide free (ab250866)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.