Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)
![Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)](https://www.abcam.com/ps/products/250/ab250247/Images/ab250247-405706-anti-rac1-rac2-rac3-antibody-epr18631-bsa-and-azide-free-western-blot.jpg "Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18631] to Rac1 + Rac2 + Rac3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free
See all Rac1 + Rac2 + Rac3 primary antibodies -
Description
Rabbit monoclonal [EPR18631] to Rac1 + Rac2 + Rac3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250247 is the carrier-free version of ab180683. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250247 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18631 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)All lanes : Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution
Lane 1 : Recombinant Human Rac1 full length protein
Lane 2 : Recombinant Human Rac2 full length protein
Lane 3 : Recombinant Human Rac3 full length protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaThis data was developed using ab180683, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-2: 1 second; Lane 3: 3 minutes.
Recombinant human Rac1 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac2 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac3 full length protein contains aa1-192 with a C-terminal His-Tag®. All three recombinant Rac's were made in house.
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![Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)](https://www.abcam.com/ps/products/250/ab250247/Images/ab250247-403474-anti-rac1-rac2-rac3-antibody-epr18631-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)All lanes : Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaThis data was developed using ab180683, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 seconds; Lanes 2-4 : 15 seconds.
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![Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)](https://www.abcam.com/ps/products/250/ab250247/Images/ab250247-399345-anti-rac1-rac2-rac3-antibody-epr18631-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)All lanes : Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 3 secondsThis data was developed using ab180683, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)](https://www.abcam.com/ps/products/250/ab250247/Images/ab250247-4-benefits-of-recombinant-antibodies.png)
Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] - BSA and Azide free (ab250247)