Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15265-21] to ARL2BP - BSA and Azide free
  • Suitable for: ICC, IP, Flow Cyt, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free
    See all ARL2BP primary antibodies

  • Description

    Rabbit monoclonal [EPR15265-21] to ARL2BP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Rat
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HUVEC, MCF7, RAW 264.7 and NIH/3T3 cell lysates; Mouse brain and heart tissue lysate; Rat spleen tissue lysate. Flow Cyt: A549 cells. ICC: A549 cells. IHC-P: Human kidney tissue.

  • General notes

    Ab250951 is the carrier-free version of ab188322. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250951 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    Western blot - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    All lanes : Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    This data was developed using ab188322, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)

    This data was developed using ab188322, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ARL2BP with ab188322 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    Western blot - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    All lanes : Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ARL2BP knockout HeLa cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 19 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab188322, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab188322 observed at 20 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab188322 Anti-ARL2BP antibody [EPR15265-21] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265269 (knockout cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab188322 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    Immunocytochemistry - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    This data was developed using ab188322, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling ARL2BP with ab188322 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counterstained with Dapi (blue).
  • Flow Cytometry - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    Flow Cytometry - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    This data was developed using ab188322, the same antibody clone in a different buffer formulation.Flow cytometry analysis of A549 (human lung carcinoma) cells labelling ARL2BP (red) with purified ab188322 at a dilution of 1/200. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.
  • Western blot - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    Western blot - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    All lanes : Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : NIH/3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    This data was developed using ab188322, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    This data was developed using ab188322, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ARL2BP with ab188322 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    This data was developed using ab188322, the same antibody clone in a different buffer formulation.Western blot analysis of ARL2BP in HepG2 cell lysate immunoprecipitated with ab188322 at 1/50 dilution (Lane 1). Lane 2: PBS instead of HepG2 lysate.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
  • Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)
    Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (ab250951)

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