Anti-p21 antibody [CIP1/823] (ab220206)
Key features and details
- Mouse monoclonal [CIP1/823] to p21
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-p21 antibody [CIP1/823]
See all p21 primary antibodies -
Description
Mouse monoclonal [CIP1/823] to p21 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human p21 aa 1 to the C-terminus.
Database link: P38936 -
Positive control
- WB: HeLa cell lysate. ICC: HeLa cells. IHC-P: Human lung squamous cell carcinoma and bladder carcinoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab220206 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
CIP1/823 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Anti-p21 antibody [CIP1/823] (ab220206) at 1 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 18 kDaWestern blot analysis of HeLa cell lysate labeling p21 with ab220206 at 1 ug/ml.
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Immunocytochemistry analysis of HeLa cells labeling p21 with ab220206 at 1 ug/ml followed by Goat anti-Mouse IgG (Red). Membrane stained with Phalloidin 488 (Green).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling p21 with ab220206 at 4 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue labeling p21 with ab220206 at 4 µg/ml.