Anti-Caspase-9 (phospho Y153) antibody (ab79202)
Key features and details
- Rabbit polyclonal to Caspase-9 (phospho Y153)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caspase-9 (phospho Y153) antibody
See all Caspase-9 primary antibodies -
Description
Rabbit polyclonal to Caspase-9 (phospho Y153) -
Host species
Rabbit -
Specificity
Detects Caspase-9 only when phosphorylated at tyrosine 153. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Caspase-9.
Database link: P55211 -
Positive control
- ICC: HeLa cells; WB: extracts from COLO205 cells; IHC-P: thymus tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-9 (phospho Y153) antibody (ab79202)All lanes : Anti-Caspase-9 (phospho Y153) antibody (ab79202) at 1/500 dilution
Lane 1 : COLO205 cell extracts
Lane 2 : COLO205 cell extracts with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Additional bands at: 40 kDa (possible cleavage fragment)
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Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 (phospho Y153) antibody (ab79202)Left panel: immunofluorescence analysis of HeLa cells using 1/500 ab89202. Right panel: same, after pre-incubation with the immunizing phosphopeptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 (phospho Y153) antibody (ab79202)Left panel: immunohistochemistry analysis of paraffin-embedded Human thymus tissue using 1/50 ab79202. Right panel: same, after pre-incubation with the immunizing phosphopeptide.
