Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µg 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Caspase-3 antibody [ABM1C12] (ab208161)

Key features and details

Mouse monoclonal [ABM1C12] to Caspase-3
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human
Isotype: IgG1

Product name

Anti-Caspase-3 antibody [ABM1C12]
See all Caspase-3 primary antibodies
Description

Mouse monoclonal [ABM1C12] to Caspase-3
Host species

Mouse
Specificity

ab208161 recognizes both pro- and active Caspase 3 after apoptosis has been induced in wildtype cells and not knockout cells
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human Caspase-3 aa 1 to the C-terminus.
Database link: P70677

Run BLAST with Run BLAST with
Positive control
- Ramos cell lysate. Human colon, adenocarcinoma of rectum, spleen and squamous cell carcinoma of lung tissues.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

ABM1C12
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161)

Lane 1: Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h)
Lane 2: Wild-type HAP1 cell lysate
Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h)
Lane 4: Caspase-3 knockout HAP1 cell lysate
Lanes 1 - 4: Merged signal (red and green). Green - ab208161 observed at 35 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab208161 was shown to recognise pro + active Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (± Staurosporine treatment) were subjected to SDS-PAGE. ab208161 at a concentration of 2 µg/ml and ab181602 (loading control to GAPDH) at a dilution of 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human adenocarcinoma of rectum tissue labeling active + pro Caspase 3 with ab208161 at 5 µg/ml .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon tissue labeling active + pro Caspase 3 with ab208161 at 5 µg/ml .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human spleen tissue labeling active + pro Caspase 3 with ab208161 at 5 µg/ml .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [ABM1C12] (ab208161)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human squamous cell carcinoma of lung tissue labeling active + pro Caspase 3 with ab208161 at 5 µg/ml .
Western blot - Anti-Caspase-3 antibody [ABM1C12] (ab208161)

Anti-Caspase-3 antibody [ABM1C12] (ab208161) at 2 µg/ml + Ramos cell lysate

Predicted band size: 31 kDa

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