Biotin Anti-Caspase-3 antibody [E87] (ab195905)
![Biotin Anti-Caspase-3 antibody [E87] (ab195905)](https://www.abcam.com/ps/products/195/ab195905/Images/ab195905-259465-IHCAP217053301.jpg "Biotin Anti-Caspase-3 antibody [E87] (ab195905)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [E87] to Caspase-3
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Conjugation: Biotin
Overview
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Product name
Biotin Anti-Caspase-3 antibody [E87]
See all Caspase-3 primary antibodies -
Description
Biotin Rabbit monoclonal [E87] to Caspase-3 -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Recombinant fragment
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Active Caspase-3 recombinant protein. IHC-P: Human normal tonsil (FFPE sections)
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E87 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Caspase-3 antibody [E87] (ab195905)
IHC image of Caspase-3 staining in a section of formalin-fixed paraffin-embedded Human normal tonsil*, performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab195905, 1/50 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Biotin Anti-Caspase-3 antibody [E87] (ab195905)](https://www.abcam.com/ps/products/195/ab195905/Images/ab195905-244681-WBab1959051.jpg)
Western blot - Biotin Anti-Caspase-3 antibody [E87] (ab195905)Biotin Anti-Caspase-3 antibody [E87] (ab195905) at 1/10000 dilution +Recombinant human Cleaved Caspase-3 protein (Active) (ab52101) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195905 overnight at 4°C. Antibody binding to the target was detected using a HRP conjugated streptavidin amplification step, and visualised using ECL development solution ab133406.
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![Biotin Anti-Caspase-3 antibody [E87] (ab195905)](https://www.abcam.com/ps/products/195/ab195905/Images/ab195905-3-benefits-of-recombinant-antibodies.png)
Biotin Anti-Caspase-3 antibody [E87] (ab195905)
