Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Caspase-3 (phospho S150) antibody (ab59425)

Key features and details

Rabbit polyclonal to Caspase-3 (phospho S150)
Suitable for: IHC-P, WB
Reacts with: Human
Isotype: IgG

Product name

Anti-Caspase-3 (phospho S150) antibody
See all Caspase-3 primary antibodies
Description

Rabbit polyclonal to Caspase-3 (phospho S150)
Host species

Rabbit
Specificity

Caspase-3 (Phospho-Ser150) antibody detects endogenous levels of Caspase-3 only when phosphorylated at serine 150.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human Caspase-3.
Database link: P42574
Positive control
- Human lymph node tissue

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab59425 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site
Clonality

Polyclonal
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 (phospho S150) antibody (ab59425)

ab59425, at a 1/50 dilution, staining paraffin embedded human lymph node tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the phosphopeptide.
Western blot - Anti-Caspase-3 (phospho S150) antibody (ab59425)

All lanes : Anti-Caspase-3 (phospho S150) antibody (ab59425)

Lane 1 : Jurkat cells, treated with Etoposide (25µM, 60mins)
Lane 2 : Jurkat cells, treated with Etoposide (25µM, 60mins) with blocking phosphopeptide.

Predicted band size: 31 kDa
Observed band size: 34 kDa
why is the actual band size different from the predicted?
Additional bands at: 23 kDa (possible isoform)

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