Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17029] to Caspase-7 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free
    See all Caspase-7 primary antibodies

  • Description

    Rabbit monoclonal [EPR17029] to Caspase-7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250510 is the carrier-free version of ab181579. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250510 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)
    Western blot - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)
    All lanes : Anti-Caspase-7 antibody [EPR17029] (ab181579) at 1/5000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : C6 (Rat glial tumor cells) cell lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 35,32,27 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab181579, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Observed band size: 35kDa band is the pro-Caspase 7, 32kDa band is the N-terminal propeptide cleaved Caspase 7, and the 27kDa band is an N-terminal truncated caspase 7.

  • Western blot - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)
    Western blot - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)
    All lanes : Anti-Caspase-7 antibody [EPR17029] (ab181579) at 1/5000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa



    This data was developed using ab181579, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Observed band size: The 35kDa band is the pro-caspase 7; 32kDa band is the N-terminal propeptide cleaved caspase 7; the 27kDa band is the truncated form and the 11kDa band is Caspase 7 subunit p11.

    Lanes 1-4 were from the same blot; the exposure time for lanes 1-3 is 1 min and for lane 4 is 2 min.

  • Immunocytochemistry - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)
    Immunocytochemistry - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)
    This data was developed using ab181579, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling Caspase-7 with ab181579 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm and nuclear staining on NIH/3T3 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab181579 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution. Please see Pubmed IDs: 22464733 and 11829465 for further information on Caspase-7 localization.
  • Immunoprecipitation - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)
    Immunoprecipitation - Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)
    This data was developed using ab181579, the same antibody clone in a different buffer formulation.Caspase-7 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) with ab181579 at 1/120 dilution. Western blot was performed from the immunoprecipitate using ab181579 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: NIH/3T3 whole cell extract. Lane 2: PBS instead of extract.Blocking/Dilution buffer: 5% NFDM/TBST.The observed band at 35kDa band is the pro-caspase-7.
  • Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)
    Anti-Caspase-7 antibody [EPR17029] - BSA and Azide free (ab250510)

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