Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22839-12] to Caspase-7 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free
    See all Caspase-7 primary antibodies

  • Description

    Rabbit monoclonal [EPR22839-12] to Caspase-7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Untreated wild-type HAP1 whole cell lysate, Wild-type HAP1 treated with 1 M staurosporine for 3 hours whole cell lysate, C6, PC-12, RAW 264.7 whole cell lysate, Untreated HeLa, HeLa treated with 1 M staurosporine for 3 hours whole cell lysate and C2C12 lysate. IHC-P: Human, mouse and rat colon tissues. Flow Cyt: HeLa and C2C12 cells.

  • General notes

    Ab256539 is the carrier-free version of ab255818. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256539 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)
    Western blot - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)
    All lanes : Anti-Caspase-7 antibody [EPR22839-12] (ab255818) at 1/1000 dilution

    Lane 1 : Untreated wild-type HAP1 whole cell lysate
    Lane 2 : Wild-type HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate
    Lane 3 : Untreated caspase-7 knockout HAP1 whole cell lysate
    Lane 4 : Caspase-7 knockout HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate
    Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 7 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 32,35 kDa
    why is the actual band size different from the predicted?



    Caspase-7 is activated upon apoptotic induction. Procapase-7 (35 kDa), intermediate caspase-7 (32 kDa) and caspase-7 p20 subunit (18 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 15953353, 18596415). 

    ab255818 was shown to specifically react with Caspase-7 in wild-type HAP1 cells as signal was lost in Caspase-7 knockout cells. Wild-type and Caspase-7 knockout samples were subjected to SDS-PAGE. ab255818 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ instrument using the ECL technique. Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255818).

  • Flow Cytometry - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)
    Flow Cytometry - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C2C12 (mouse muscle) cells labeling Caspase-7 with ab255818 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255818).

  • Flow Cytometry - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)
    Flow Cytometry - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma) cells labeling Caspase-7 with ab255818 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255818).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Caspase-7 with ab255818 at 1/2000 dilution (0.28 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat colon (PMID: 30448733) is observed. The section was incubated with ab255818 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255818).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Caspase-7 with ab255818 at 1/2000 dilution (0.28 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse colon (PMID: 23572575) is observed. The section was incubated with ab255818 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255818).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Caspase-7 with ab255818 at 1/2000 dilution (0.28 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon (PMID: 30448733) is observed. The section was incubated with ab255818 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255818).

  • Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)
    Anti-Caspase-7 antibody [EPR22839-12] - BSA and Azide free (ab256539)

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