HRP Anti-Caspase-7 antibody [E22] (ab206039)
![HRP Anti-Caspase-7 antibody [E22] (ab206039)](https://www.abcam.com/ps/products/206/ab206039/Images/ab206039-305133-ab206039HRPKOWBJRIZ.jpg "HRP Anti-Caspase-7 antibody [E22] (ab206039)")
Key features and details
- HRP Rabbit monoclonal [E22] to Caspase-7
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Caspase-7 antibody [E22]
See all Caspase-7 primary antibodies -
Description
HRP Rabbit monoclonal [E22] to Caspase-7 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Caspase-7 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P55210 -
Positive control
- WB: Jurkat whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E22 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Caspase-7 antibody [E22] (ab206039)All lanes : HRP Anti-Caspase-7 antibody [E22] (ab206039) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CASP7 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesab206039 was shown to specifically react with Caspase-7 in wild-type HAP1 cells as signal was lost in CASP7 knockout cells. Wild-type and CASP7 knockout samples were subjected to SDS-PAGE. Ab206039 was incubated overnight at 4°C at 1/5000 dilution. Blots were developed with ECL technique.
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![Western blot - HRP Anti-Caspase-7 antibody [E22] (ab206039)](https://www.abcam.com/ps/products/206/ab206039/Images/ab206039-255255-WBab2060391.jpg)
Western blot - HRP Anti-Caspase-7 antibody [E22] (ab206039)HRP Anti-Caspase-7 antibody [E22] (ab206039) at 1/5000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab206039 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
