Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-7 antibody [7-1-11] (ab69540)

Key features and details

  • Mouse monoclonal [7-1-11] to Caspase-7
  • Suitable for: ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Caspase-7 antibody [7-1-11]
    See all Caspase-7 primary antibodies

  • Description

    Mouse monoclonal [7-1-11] to Caspase-7

  • Host species

    Mouse

  • Specificity

    This antibody recognizes the unprocessed pro caspase-7 (p35), caspase-7 lacking the short aminoterminal pro domain (32 kDa) and the fully processed caspase-7 (p19) in apoptotic cell extracts.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human Caspase-7.
    Database link: P55210

  • Positive control

    • HeLa Cell Lysate Rat Brain Tissue Extract Jurkat cell lysate treated with staurosporine IF/ICC: MCF7 cell line.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [7-1-11] (ab69540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [7-1-11] (ab69540)

    ab69540, at 10 µg/ml, staining Caspase-7 in formalin fixed, paraffin embedded Human spleen tissue by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen were used to develop staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [7-1-11] (ab69540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [7-1-11] (ab69540)

    ab69540, at 10 µg/ml, staining Caspase-7 in formalin fixed, paraffin embedded Human colon tissue by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen were used to develop staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [7-1-11] (ab69540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [7-1-11] (ab69540)

    ab69540, at 10 µg/ml, staining Caspase-7 in formalin fixed, paraffin embedded Human placenta tissue by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen were used to develop staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [7-1-11] (ab69540)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [7-1-11] (ab69540)

    ICC/IF image of ab69540 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69540, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-Caspase-7 antibody [7-1-11] (ab69540)
    Flow Cytometry - Anti-Caspase-7 antibody [7-1-11] (ab69540)

    Overlay histogram showing Jurkat cells stained with ab69540 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab69540, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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