Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-AF10 antibody [EPR20810] (ab208016)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20810] to AF10
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-AF10 antibody [EPR20810]
See all AF10 primary antibodies
Description

Rabbit monoclonal [EPR20810] to AF10
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562 and ES-D3 whole cell lysates; Mouse and rat testis lysates. IHC-P: Human, mouse and rat testis tissues. Flow Cyt: K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20810
Isotype

IgG
Research areas

Western blot - Anti-AF10 antibody [EPR20810] (ab208016)

Lanes 1-3 : Anti-AF10 antibody [EPR20810] (ab208016) at 1/5000 dilution
Lane 4 : Anti-AF10 antibody [EPR20810] (ab208016) at 1/1000 dilution

Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : ES-D3 (mouse embryonic multipotent stem cell line) whole cell lysate
Lane 3 : Mouse testis lysate
Lane 4 : Rat testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 113 kDa
Observed band size: 130 kDa
why is the actual band size different from the predicted?

Exposure time : Lanes 1-2: 3 minutes; Lane 3: 5 seconds; Lane 4: 15 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] (ab208016)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling AF10 with ab208016 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human testis (PMID: 7888665; PMID: 11756182). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using Tris/EDTA buffer, pH 9.0.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] (ab208016)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling AF10 with ab208016 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse testis (PMID: 7888665; PMID: 11756182). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using Tris/EDTA buffer, pH 9.0.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] (ab208016)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling AF10 with ab208016 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in rat testis (PMID: 7888665; PMID: 11756182). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using Tris/EDTA buffer, pH 9.0.
Flow Cytometry - Anti-AF10 antibody [EPR20810] (ab208016)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling AF10 with ab208016 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-AF10 antibody [EPR20810] (ab208016)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com