Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20810] to AF10 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AF10 antibody [EPR20810] - BSA and Azide free
    See all AF10 primary antibodies

  • Description

    Rabbit monoclonal [EPR20810] to AF10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human testis tissue.

  • General notes

    Ab230376 is the carrier-free version of ab208016. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230376 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)
    Flow Cytometry - Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling AF10 with ab208016 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208016).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling AF10 with ab208016 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in rat testis (PMID: 7888665; PMID: 11756182). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using Tris/EDTA buffer, pH 9.0.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208016).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling AF10 with ab208016 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse testis (PMID: 7888665; PMID: 11756182). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using Tris/EDTA buffer, pH 9.0.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208016).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling AF10 with ab208016 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human testis (PMID: 7888665; PMID: 11756182). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208016).

  • Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)
    Anti-AF10 antibody [EPR20810] - BSA and Azide free (ab230376)

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