Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Fos B antibody [EPR15905] (ab184938)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15905] to Fos B
Suitable for: IP, ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Fos B antibody [EPR15905]
See all Fos B primary antibodies
Description

Rabbit monoclonal [EPR15905] to Fos B
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Rat
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr; NIH/3T3 and C6 whole cell lysate treated with serum, starved overnight, then serum stimulated for 4hr. IHC-P: Mouse hippocampus, mouse kidney, rat hippocampus and rat kidney tissues. ICC/IF: HeLa cells. IP: HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15905
Isotype

IgG
Research areas

Western blot - Anti-Fos B antibody [EPR15905] (ab184938)

All lanes : Anti-Fos B antibody [EPR15905] (ab184938) at 1/10000 dilution

Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 38,48 kDa
why is the actual band size different from the predicted?

Exposure time: 5 seconds

FosB protein exists as two isoforms: full-length FosB (48KD) and a truncated form, FosB2 (38KD, Delta FosB) which lacks the carboxyl-terminal 101 amino acids.
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Fos B antibody [EPR15905] (ab184938)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, untreated or treated with 12-o-tetradecanoyl phorbol 13-acetate (TPA) (200nM) for 4h, labeling Fos B with ab184938 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cells. The expression was increaed after treatment with 12-o-tetradecanoyl phorbol 13-acetate (TPA) (200nM) for 4h.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184938 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-Fos B antibody [EPR15905] (ab184938)

All lanes : Anti-Fos B antibody [EPR15905] (ab184938) at 1/10000 dilution

Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate treated with serum, starved overnight, then serum stimulated for 4hr

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 38,48 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
FosB protein exists as two isoforms: full-length FosB (48KD) and a truncated form, FosB2 (38KD, Delta FosB) which lacks the carboxyl-terminal 101 amino acids
Western blot - Anti-Fos B antibody [EPR15905] (ab184938)

All lanes : Anti-Fos B antibody [EPR15905] (ab184938) at 1/10000 dilution

Lane 1 : Untreated C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : C6 whole cell lysate treated with serum, starved overnight, then serum stimulated for 4hr

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 38,48 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
FosB protein exists as two isoforms: full-length FosB (48KD) and a truncated form, FosB2 (38KD, Delta FosB) which lacks the carboxyl-terminal 101 amino acids
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse hippocampus is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse kidney is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat hippocampus is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR15905] (ab184938)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Fos B with ab184938 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on rat kidney is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Fos B antibody [EPR15905] (ab184938)

Fos B was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr using ab184938 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab184938 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr, 10 µg (Input).
Lane 2: ab184938 IP in HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184938 in HeLa whole cell lysate serum starved overnight, then treated with 200nM 12-o-tetradecanoyl phorbol 13-acetate (TPA) for 4hr.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-Fos B antibody [EPR15905] (ab184938)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com