Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23489-90] to Fos B - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, IP, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Fos B antibody [EPR23489-90] - BSA and Azide free
    See all Fos B primary antibodies

  • Description

    Rabbit monoclonal [EPR23489-90] to Fos B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB & IP: HeLa whole cell lysate, serum starved o/n, then treated with 200 nM 12-o-tetradecanoyl phorbol 13-acetate for 4 hrs. IHC-P: Human breast cancer and hippocampus tissue. ICC/IF: HeLa cells, starved o/n, then treated with 200 nM 12-o-tetradecanoyl phorbol 13-acetate for 4 hrs. Flow Cyt: HeLa cells, serum starved o/n, then treated with 200 nM 12-o-tetradecanoyl phorbol 13-acetate for 4 hrs.

  • General notes

    ab269953 is the carrier-free version of ab252237. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)
    Flow Cytometry - Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) was serum starved for 16 hours, then treated with 200nM 12-O-Tetradecanoylphorbol-13-acetate (TPA) for 4 h (Red) / Untreated control (Green) cells labeling Fos B with ab252237 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252237).

  • Immunocytochemistry/ Immunofluorescence - Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)
    Immunocytochemistry/ Immunofluorescence - Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Fos B with ab252237 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells treated with starvation 16 hours, then 12-O-Tetradecanoylphorbol-13-acetate (200nM) for 4 hours. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252237).

  • Immunoprecipitation - Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)
    Immunoprecipitation - Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)

    Fos B was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) serum starved overnight, then treated with 200 nM TPA for 4 hours, whole cell lysate 10 µg with ab252237 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab252237 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa serum starved overnight, then treated with 200 nM TPA for 4 hours, whole cell lysate 10 µg.

    Lane 2: ab252237 IP in HeLa serum starved overnight, then treated with 200 nM TPA for 4 hours, whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252237 in HeLa serum starved overnight, then treated with 200 nM TPA for 4 hours, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252237).

    The band around 37kDa is caused by splicing isoform.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Fos B with ab252237 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive nuclear staining in cancer cells of human breast cancer is observed (PMID: 12602926). The section was incubated with ab252237 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252237).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)

    Immunohistochemical analysis of paraffin-embedded human hippocampus tissue labeling Fos B with ab252237 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive nuclear staining in neurons of human hippocampus is observed (PMID: 29805976). The section was incubated with ab252237 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252237).

  • Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)
    Anti-Fos B antibody [EPR23489-90] - BSA and Azide free (ab269953)

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