Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20463] to NFE2 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NFE2 antibody [EPR20463] - BSA and Azide free
    See all NFE2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20463] to NFE2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251577 is the carrier-free version of ab223845 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251577 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Nuclear Factor Erythroid Derived 2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
    Western blot - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
    All lanes : Anti-NFE2 antibody [EPR20463] (ab223845) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : HEL (human bone marrow erythroleukemia cell line) whole cell lysate
    Lane 3 : TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 3 minutes


    This data was developed using ab223845, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
    This data was developed using ab223845, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling NFE2 with ab223845 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in neutrophils (arrow) in human placenta (PMID: 8645254) is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
    This data was developed using ab223845, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded malignant lymphoma of bone marrow tissue labeling NFE2 with ab223845 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on a subset of cells of malignant lymphoma of bone marrow is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
    Flow Cytometry - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
    This data was developed using ab223845, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEL (human bone marrow erythroleukemia) cell line labeling NFE2 with ab223845 at 1/400 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
  • Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
    Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)

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