Anti-Caspase-7 antibody (ab92842)
Key features and details
- Rabbit polyclonal to Caspase-7
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caspase-7 antibody
See all Caspase-7 primary antibodies -
Description
Rabbit polyclonal to Caspase-7 -
Host species
Rabbit -
Specificity
The immunogen used for this product detects both caspase-7 precursor and small 105 aa C-terminal p12/12 kDa subunit in staurosporine (STS) treated cells. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
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Positive control
- This antibody gave a positive signal in the following lysates: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Protein A purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanApplication Abreviews Notes WB Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death. -
Tissue specificity
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. -
Sequence similarities
Belongs to the peptidase C14A family. -
Post-translational
modificationsCleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
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Alternative names
- Apoptotic protease Mch-3 antibody
- Apoptotic protease MCH3 antibody
- CASP-7 antibody
see all
Images
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Western blot - Anti-Caspase-7 antibody (ab92842)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Wild-type HAP1 treated with Staurosporin whole cell lysate (20 µg)
Lane 3: Caspase-7 knockout HAP1 whole cell lysate (20 µg)
Lane 4: Caspase-7 knockout HAP1 treated with Staurosporin whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: HeLa treated with Staurosporin whole cell lysate (20 µg)Lanes 1 - 6: Merged signal (red and green). Green - ab92842 observed at 37, 12 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab92842 was shown to recognize Caspase-7 in wild-type cells as signal was lost at the expected MW in Caspase-7 knockout cells. Additional cross-reactive bands were observed in the wild-type and Caspase-7 knockout cells. Wild-type and Caspase-7 knockout samples were subjected to SDS-PAGE. Ab92842 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Caspase-7 antibody (ab92842)All lanes : Anti-Caspase-7 antibody (ab92842) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesWe hypothesize that the 15 kDa band represents the cleaved subunit (P11) of Caspase-7. Abcam welcomes customer feedback.
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Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody (ab92842)ICC/IF image of ab92845 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92842, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1:1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab92842 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Caspase-7 antibody (ab92842)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Wild-type HAP1 treated with Staurosporin whole cell lysate (20 µg)
Lane 3: Caspase-7 knockout HAP1 whole cell lysate (20 µg)
Lane 4: Caspase-7 knockout HAP1 treated with Staurosporin whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: HeLa treated with Staurosporin whole cell lysate (20 µg)Lanes 1 - 6: Merged signal (red and green). Green - ab92842 observed at 37, 12 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab92842 was shown to recognize Caspase-7 in wild-type cells as signal was lost at the expected MW in Caspase-7 knockout cells. Additional cross-reactive bands were observed in the wild-type and Caspase-7 knockout cells. Wild-type and Caspase-7 knockout samples were subjected to SDS-PAGE. Ab92842 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Caspase-7 antibody (ab92842)All lanes : Anti-Caspase-7 antibody (ab92842) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesWe hypothesize that the 15 kDa band represents the cleaved subunit (P11) of Caspase-7. Abcam welcomes customer feedback.
-
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody (ab92842)
ICC/IF image of ab92845 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92842, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1:1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.
