Anti-ATF6 antibody (ab203119)
Key features and details
- Rabbit polyclonal to ATF6
- Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATF6 antibody
See all ATF6 primary antibodies -
Description
Rabbit polyclonal to ATF6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ATF6 aa 300-400 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P18850 -
Positive control
- WB: huvec, raji, jurkat, a549, thp-1, 293t and du145 cells ICC/IF: U-251 cells IHC: HeLa cells Flow-Cyt: Jurkat cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.01% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATF6 antibody (ab203119)Anti-ATF6 antibody (ab203119) at 1/300 dilution + mouse pancreas lysates
Secondary
Conjugated secondary antibody at 1/10000 dilution
Predicted band size: 75 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF6 antibody (ab203119)Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat brain tissue sections labeling ATF6 with ab203119 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-ATF6 antibody (ab203119)Immunocytochemistry/ Immunofluorescence analysis of U-251 cells labeling ATF6 with ab203119 at 1/200 (diluted in PBS and incubated for 2 hours at 37°C). Goat Anti-Rabbit IgG (H+L) FITC conjugated antibody was used as the secondary antibody. Nuclei were stained with DAPI (blue).
